Quantification Of Faecal Microbiota By Rt
Fresh faecal samples were taken in the fourth week of feeding the experimental diets and stored at 20°C until DNA isolation. Genomic DNA was extracted with 700 l DNA extraction buffer from 70 mg frozen faeces with mechanical disruption ) using a QIAamp DNA stool mini kit . DNA quality was verified on an agarose gel and the concentration was quantified using a NanoPhotometer . Quantitative PCR was performed using the oligonucleotide primers . One PCR reaction was performed with 2 l of diluted DNA , 05 l of each primer , 6 l 2× buffer SensiFAST SYBR No-ROX mix and 3 l H2O PCR grade. Amplifications were detected on a Light Cycler 96 . One primer pair was designed to detect the bacterial 16S rRNA gene, and its amount was utilised to normalise amplicon expression. The efficiency of amplification was calculated using LinRegPCR software, version 2014.4 , yielding efficiency values were between 175 and 185 . The genus Lactobacillus was reclassified in March 2020. However, the primers used to detect all Lactobacillaceae classified as such until 2020. Data were quantified by qbasePlus software .
Kos Organic Plant Protein
Each flavor of this KOS vegan protein powder provides its own unique benefits on top of weight gain support and healthy muscle growth.
Blueberry muffin comes with a signature immunity support blend and digestive enzyme blend.
The chocolate chip mint also has digestive enzyme support plus a fruit & veggie blend and signature vitamins & minerals blend.
Oh, and if flavor is king for you, try their Salted Caramel Coffee – it has a gold award for its fantastic taste and benefits!
This protein powder has 155 calories per serving with 25g of protein.
You can get all flavors here – they offer classic vanilla, chocolate, and peanut butter flavors along with the salted caramel coffee.
Optimum Nutrition Serious Mass
If you’re looking for a protein powder that offers more flavors but still has all of the protein and calories you need, Serious Mass from Optimum Nutrition is an excellent choice.
Every 2 scoops have 1,250 calories with 50g of protein! And it comes in five delicious flavors – banana, chocolate, chocolate peanut butter, strawberry, and vanilla.
You can pick from all of the flavors here.
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Foods And Beverages To Consume In Limited Quantities
The foods on this list can be included, but they shouldnt be the main focus if you want to lose weight. Although many of them are nutritious, their protein percentages arent high. Depending on what you like and the way you eat, you can enjoy them in small quantities on a frequent or even daily basis.
- Cheese: Cheese is delicious, low in carbs, and a great source of calcium. However, it doesnt provide a lot of protein per calorie. Additionally, some people find that eating cheese causes cravings for more.22If you follow a keto or low-carb diet, feel free to have a small portion daily, if you like.
Protein percentage range: 39% to 20%
- Nuts: They provide crunch, flavor, and enjoyment at meals and when snacking. Unfortunately, they dont provide much nutrition per calorie.Also, once you start eating nuts, it can be difficult to stop. Eating too many nuts can increase your caloric intake, making weight loss more difficult.23Aim for no more than a small handful no matter what type of nut you choose. Also, portion them out and eat them in a small bowl rather than from the container.
Protein percentage range: 18% to 4%
- Low-protein processed meats: Yes, theyre flavorful and convenient. But foods like bacon, sausage, and salami contain far less protein than other meats. Can you still lose weight if you include them in your diet? Absolutely. But try to choose the processed meats listed in the foods to eat section most of the time.
Protein percentage range: 23% to 14%
Gestating And Lactating Dogs
A gestating dogs diet should be high in protein. For lactating dogs, protein is just one factor to consider. A lactating dog needs a high-quality, nutrient-dense diet that is also high in calories. Before deciding on a food, its wise to consult your veterinarian to be sure your gestating or lactating dog will be getting adequate protein, fat and calories during this critical time.
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Energy Expenditure And Nutrient Oxidation
In the fourth week of feeding the experimental diets, mice were adapted for a period of 2 h on two different days to the respiration chambers before indirect calorimetry measurements. After acclimation, mice were placed in the respiration chambers with 20 g of the experimental diets and 50 ml of water. Indirect calorimetry was performed at 22°C with 12:12 h darklight cycle to measure total energy expenditure and carbohydrate and fat oxidation as previously described. Physical activity was recorded by an infrared motion detector converting movements into the numbers of impulses per time interval . Data were collected using Simatic hardware and Win CC software . Food and water intake were recorded over 48 h. The body weight was determined before and after gas exchange measurements to calculate the mean body weight. The daily total energy expenditure was calculated according to Weir: TEE = 1629 × VO2 + 457 × VCO2, where VO2 is the oxygen consumption and VCO2 is the CO2 production . Net oxidation rates of fat and carbohydrates were calculated according to Simonson and DeFronzo: FOX = 169 × and COX = 457 × VCO2 323 × VO2. The respiratory quotient was calculated as the ratio of VCO2 to VO2, and the daily means for the 48-h measuring period were calculated. The energy balance was calculated: EB = energy intake faecal energy TEE . One animal per group was removed from the evaluation because of technical problems with the respiration chambers.
/7so How Much Protein Should You Have In A Day
According to the Mayo Clinic, anywhere from 10% to 35% of your calories should come from protein. So if your body requires 2,000 calories, then you must aim for 200â700 calories from protein, or 50â175 grams, explains the health body. 0.8 grams of protein per kilo of one’s body weight is the recommended dietary allowance to prevent deficiency in any average physically inactive adult.
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Does Protein Make You Gain Weight What You Need To Know
How to use protein to meet your weight goals.
No doubt about it, protein is a nutritional powerhouse.
Like the other macronutrients, carbohydrates and fat, protein plays a unique and vital role in both your overall health and your day-to-day functioning. Its essential for building and maintaining muscle and other tissues, like bones, skin, and other organs.
And, protein isnt just for bodybuilders. Even if youre not actively trying to build muscle, getting adequate protein ensures that the muscle you have stays healthy.
The Dietary Guidelines for Americans recommend that the average person consume 10 to 35 percent of total calories from protein, and the minimum recommended amount is 0.8 grams per kilogram of bodyweight for a 185-pound man, thats just 67 grams per day.
However, as Mens Health previous reported, consuming 20 to 30 percent of your calories from protein, which breaks down to about 1.2 to 1.6 grams per kilogram, is a better goal. Thats between 90 and 120 grams of protein if you weigh 185 pounds.
But what about protein and weight gain? Because protein contains calories , it stands to reason that eating more protein can increase your calorie intake overall. And, consuming more calories than you burn each day is what leads to weight gain. That said, will increased protein consumption lead to weight gain?
We have answers.
What You Can Eat
A high-protein diet generally recommends getting more than 20% of your total calories from protein. That typically means eating fewer calories from carbohydrates or fats to keep your calorie total in balance.
No foods are expressly forbidden on a high protein diet, but eating more lean proteins and fewer refined carbohydrates, sugars, and fats are recommended.
There is no suggested meal timing for a high-protein diet. However, some people on a high-protein plan also practice intermittent fasting, which involves restricting calories to specific days of the week and fasting on others, or going for extended periods without eating each day, such as 16 hours a day.
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Risks To Heart Health
Relating to diets heavily weighted in high-fat dairy and red meats, both of which are naturally high in protein, are not the best for our cardiovascular health. Due to the higher intake of cholesterol and saturated fat through such foods, there are increased chances of heart disease.
That said, eating foods such as chicken, fish, and nuts – themselves high in protein – has been shown to lower the risk of heart disease .
How Food Helps Build Muscle
Although protein is essential for muscle building, it’s not the only important food component in the process. According to the National Academy of Sports Medicine, a good muscle-building diet must also include plenty of carbohydrates, vitamins and minerals. A balanced diet that features all these will provide the necessary ingredients to help your muscles repair and rebuild after a workout.
Overall, the NASM recommends that most people consume at least 0.7 to 0.8 grams of protein and 1.8 to 3.2 grams of carbohydrates per pound of body weight each day. Total calorie intake also plays a role in muscle building, as it’s difficult to build muscle if you’re burning more calories than you consume.
Diet alone isn’t enough to stimulate muscle growth, though. Exercise especially resistance training such as lifting weights is necessary to start the process. When you tear the fibers in your muscles, that stimulates cellular growth and repair. That’s when your body can use the food you’ve eaten to rebuild and strengthen muscle tissue. Other factors also influence how effective and efficient this process is, from when you eat your food and how much rest you give your muscles between workouts to your hormonal and genetic makeup.
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What Is Protein And Why Is It Important
Protein is one of the three macronutrients, along with carbs and fat.
In your body, it performs the following roles:
- Repair and maintenance: Protein is the main component of your muscles, bones, skin and hair. These tissues are continuously repaired and replaced with new protein.
- Hormones: Chemical messenger proteins allow cells and organs in your body to communicate with each other.
- Enzymes: Most enzymes are proteins, and the thousands of chemical reactions that take place throughout your body are driven by them.
- Transportation and storage: Some proteins help deliver important molecules where theyre needed. For example, the protein hemoglobin carries oxygen to your bodys cells.
Protein is made up of smaller units known as amino acids.
Of the 22 amino acids, 9 are considered essential, meaning they must be consumed in food because your body cant make them.
Importantly, some foods provide better protein than others based on their amino acid profile.
Generally speaking, animal products are considered complete protein because they contain all the essential amino acids in the optimal amounts that your body needs. These include eggs, dairy, meat, fish and poultry.
Vegetable proteins dont provide adequate amounts of every essential amino acid but can be combined with other plant sources to make complete protein. Beans, legumes, grains, soy, nuts and seeds are examples of high-protein plant foods.
Can Protein Intake Lower Hunger Levels
Like our point on food cravings above, protein consumption naturally reduces the appetite due to protein being the most filling macronutrient.
One study found that by increasing the percentage of calories you consume from protein-rich foods, you can reduce your appetite and ultimately eat less. In this study, the overweight women ate some 441 fewer calories by increasing their protein intake .
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Fruit Juice And Weight
Read more on The Nutrition Source about the amount of sugar in soda, fruit juice, sports drinks, and energy drinks, and download the How Sweet Is It? guide to healthier beverages
Its important to note that fruit juices are not a better option for weight control than sugar-sweetened beverages. Ounce for ounce, fruit juices-even those that are 100 percent fruit juice, with no added sugar- are as high in sugar and calories as sugary sodas. So its no surprise that a recent Harvard School of Public Health study, which tracked the diet and lifestyle habits of 120,000 men and women for up to 20 years, found that people who increased their intake of fruit juice gained more weight over time than people who did not. Pediatricians and public health advocates recommend that children and adults limit fruit juice to just a small glass a day, if they consume it at all.
Alcohol and Weight
Read more about alcoholon The Nutrition Source
Even though most alcoholic beverages have more calories per ounce than sugar-sweetened beverages, theres no clear-cut evidence that moderate drinking contributes to weight gain. While the recent diet and lifestyle change study found that people who increased their alcohol intake gained more weight over time, the findings varied by type of alcohol. In most previous prospective studies, there was no difference in weight gain over time between light-to-moderate drinkers and nondrinkers, or the light-to-moderate drinkers gained less weight than nondrinkers.
High Protein Foods For Weight Gain
The phrase high protein foods for weight gain can have very different meanings to different people. Were going to focus on healthy and safe weight gain for everyone though, rather than just a certain subset of the population . We all want to put on some muscle and gain weight, but with so many protein supplements and high protein foods out there it can sometimes seem like even the experts dont know which ones are best for weight gain. After years of trial and error and trying just about everything Im going to share what Ive discovered.
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/6cutting Too Many Carbs May Lead To Binge Eating
Protein is an essential micronutrient but sufficient intake of carbs and fat are equally important. Every nutrient has a fixed role to play in the body and by cutting them from your diet you may have to face some unpleasant consequences. Insufficient carb intake may affect your mood and increase the craving for unhealthy foods, which can lead to binge eating. Thats because glucose is the preferred source of energy for our body, which is derived from carbs. When the brain finally gets carbs it craves more for it and there are high chances that one may overdo it.
Try Shakes And Supplements
If youre still struggling to meet your daily calorie goals, protein shakes and supplements can help add extra calories to your daily intake.
Meal replacement shakes tend to be higher in calories and are aimed at maintaining weight for people who cant consume regular food. Also, some protein shakes are formulated specifically for building body mass.
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Quinoa Mac And Cheese
Macaroni and cheese is love. Most people love the gooey, creamy comfort food that mac and cheese are. But when you want muscle growth, that isnt the best choice of food. Or is it?
Although mac and cheese may not find a place on a bodybuilding diet, a twist in the recipe can do the trick. Use cubed butternut squash, quinoa mac and cheese, and almond milk to prepare an easy comfort food when you are too tired of a fixed diet plan.
Summing Up
Before you jump into adding more proteins to your diet plan, theres something that you should know. Increasing protein consumption can initially lead to weight loss. Consuming more proteins helps supply the necessary building blocks to build muscle.
As you gain muscles, the body requires more energy. This leads to calorie burning and, ultimately, fat burning. Moreover, as you work out, more calories are burnt, and you gradually lose weight.
However, theres nothing to worry about here. Once you have lost the deposited fat, your body weight will stabilize. Consuming high amounts of protein will help the body to continue muscle building. With proper workouts, these muscles will shape out as you want and you will, eventually, gain weight.
So, high protein meals can help you gain weight, but the change will not be instantaneous. Moreover, you will have to work out regularly to ensure that you burn the extra calories you take along with the proteins. See you next time. Happy eating!
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Reasons You Should Be Eating Curd Daily
Dr. Vinima Mahajan
Post Graduate Diploma in Nutrition Dietetics
Dietitian/Nutritionist, panchkula 17 Years experience
Curd is a staple in every household in India whether it is served with piping hot parathas or by itself after a meal. The simple food item is packed with a number of nutrients and helps in increasing the health benefits in your body.
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Amazing Grass Vegan Protein & Kale Powder
So, I’m sure you already know all the amazing benefits of kale. It boosts nutrition and can also improve muscle function and blood flow, resulting in even more benefits from your regular physical activity.
Well, combine that with the weight and muscle gain of plant-based protein and you’ve got a match made in heaven.
For the maximum benefits of this vegan protein powder for gaining weight, be sure to choose the honey roasted peanut flavor. It has 180 calories and 20g of protein per scoop, which is about 50 more calories than the other flavors.
Get this superfood protein powder here.
Factor In Your Activity Level
Depending on how much you exercise, you’ll want to take your resting metabolic rate and multiply it by the following numbers to get a more accurate estimate of how many calories you burn on a daily basis.
- Lightly active : x 1.2
- Moderately active : x 1.4
- Very active : x 1.6
- Extra active : x 1.8
- Super active : x 1.9
For example:
1,800 calories/day x 1.2 = 2,160 calories/day
This is how many calories you need to eat to maintain your current weight if your activity level stays the same.
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